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Background: This study compared orthopedic physiotherapists from different working institutions regarding their health status and burnout syndrome to understand the work-related factors that affect health status and burnout syndrome Methods: This study was conducted from October 28 to November 13, 2022, focusing on physiotherapists working in medical institutions located in metropolitan areas, such as Seoul, Gyeonggi, and Incheon. A total of 591 questionnaires were analyzed. The questionnaire consisted of questions related to the basics of physical therapy, general matters, types of working institutions, health conditions, and burnout syndrome. Results: No significant difference was detected in health self-awareness status whether the working institution was a clinic, nursing hospital, hospital, oriental hospital, general hospital, or university hospital. Among the burnout factors, nursing hospitals showed the highest scores for retirement demands, work unfavorability, physical fatigue, mental fatigue, and reasons for work because of salary. By contrast, university hospitals showed the highest enthusiasm and other institutions showed the lowest enthusiasm for work. In particular, in terms of enthusiasm, scores were significantly more positive in university hospitals than in other institutions. Conclusion: A management plan for burnout for orthopedic manual physiotherapists working in nursing hospitals is needed, and future research will examine the health status of orthopedic manual physiotherapists and work that may affect burnout syndrome. An exploration of management measures, such as health promotion and burnout syndrome prevention, is needed that adds other items, such as differences in environment and differences in the number and disease severity of patients