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Purpose: The purpose of this study is to investigate the impact of paramedic students' five-factor personality characteristics and stress coping behavior on the stress of emergency room practice. Methods: This study conducted a survey targeting 2 grade - 4 grade paramedic students who had experience practice at emergency room at N University and B University located in Cheonan, South Chungcheong Province. Results: ‘Agreeableness’ was the highest at 33.88±6.31 and ‘Neuroticism’ was the lowest at 27.82±7.43. Among stress coping methods, ‘active coping’ was higher at 3.39 than ‘passive coping’ at 3.21, and the subarea with the highest score was ‘wishful coping’. When looking at clinical practice stress by sub-domain, ‘practical training environment’ was the highest at 2.96, and ‘interpersonal conflict’ was the lowest at 2.14. There was a negative correlation between clinical practice stress and friendliness (r=-.220, p=.014) and a positive correlation with active coping (r=.210, p=.019). The influential factors were friendliness (β=-.267) and active coping (β=.258). Conclusion: The paramedic students who participated in this study showed a high level of agreeableness among the five personality traits and used a active coping style to cope with stress. Agreeableness has been shown to lower clinical practice stress, and active coping is classified as the most appropriate coping style, so research is needed to guide people to utilize friendliness and active coping well.