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Purpose: This study aimed to identify the effects of musculoskeletal symptoms and burden on presenteeism among nurses in a gastrointestinal endoscopy unit. Methods: This was an observational cross-sectional study. Data were collected through self-reported questionnaires administered to 140 nurses working in the gastrointestinal endoscopy unit of a hospital located in Busan metropolitan city. Results: The body part with the most musculoskeletal symptoms was the back (73.2%), and the most common musculoskeletal burden work was "when you have to stand or maintain the same posture for a long time in a lead apron protection clothes.” The factors most related to work impairment were working hours, musculoskeletal symptoms, and musculoskeletal burden, with an explanatory power of 63.3%. Factors affecting perceived productivity were working hours and musculoskeletal symptoms, with an explanatory power of 29.2 %. Conclusion: To reduce work impairment and increase the perceived productivity of nurses in gastrointestinal endoscopy units, various programs and improved working environments are needed that can improve musculoskeletal symptoms and reduce musculoskeletal burden.