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Objectives: This study used raw data from the National Health and Nutrition Examination Survey to determine the effect of oral care product use on the number of existing natural teeth among middle-aged and elderly people. Methods: A total of 4,208 people, comprising2,513 middle-aged and 1,695 elderly people, were surveyed using IBM SPSS for data analysis. Results: The more middle-aged and elderly people used dental floss and the less they used other oral aids, the greater the number of existing natural teeth they possessed. Among the elderly, the number of existing natural teeth increased with interdental brushes and electric toothbrushes. Conclusions: To increase the number of existing natural teeth, regular dental checkups and professional oral health education are necessary so that middle-aged and elder can select and use oral care products suited to their oral health status.