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Purpose: This study focuses on intervention of the sling exercise combined with transcranial direct current stimulation to examine its effect on trunk muscle activity and game performance ability of soccer players. Methods: The study included 30 college soccer players and divided them into a sling exercise group (Group Ⅰ), which combines transcranial direct current stimulation, and a general sling exercise group (Group Ⅱ). Fifteen subjects were randomly assigned to each group. After receiving general soccer training, the subjects participated in a sling exercise combined with transcranial direct current stimulation (Group Ⅰ), and a general sling exercise (Group Ⅱ) for 30 min twice a week for eight weeks. Prior to intervention, the trunk muscle activity of the body (erector spinae, rectus abdominis, external oblique abdominal muscle) and the ability to perform the game (30 m sprint test and t-test) were analyzed. Eight weeks after the intervention, the above items were equally re-measured and an inter-group analysis was implemented. Results: As a result of a comparative analysis of trunk muscle activity and game performance ability between the groups, the sling exercise group (Group Ⅰ), which combined transcranial direct current stimulation, represented a statistically significant difference in trunk muscle activity and game performance ability compared to the general sling exercise group (Group Ⅱ). Conclusion: We discovered that the sling exercise, which combines transcranial direct current stimulation, is effective in improving body muscle activity and game performance ability. The use of transcranial direct current stimulation will activate muscles and improve exercise ability, which can be applied in training in various ways.