원문정보
초록
영어
Isolation of pollutant-degrading bacteria is important in bioaugmentation, one of the methods for biological degradation of environmental contaminants. We focused on the magnetic activated sludge (MAS) process as a culture method that efficiently concentrates degrading bacteria, and cultured activated sludge with 1,4-dioxane as a model pollutant. After 860 days of operation, MLVSS, which indicates the amount of sludge, increased from 390 mg/L to 10,000 mg/L, and the removal rate of organic matter including 1,4-dioxane, tetrahydrofuran, and glucose in the artificial wastewater reached up to 97%. Based on these results, the MAS process was successfully used to acclimate activated sludge with 1,4-dioxane. Bacterial flora analysis in the MAS showed that bacteria of the genus Pseudonocardia, already reported as 1,4-dioxane degrading bacteria, play an important role in the degradation of this pollutant. The MAS process is a suitable culture method for acclimation of environmental pollutants, and the findings indicate that it can be used as an enrichment unit for pollutant-degrading bacteria.
목차
1. INTRODUCTION
2. EXPERIMENTAL PROCEDURES
2.1. Experimental Apparatus
2.2.Analysis
2.3.16S rRNA gene amplicon analysis
2.4.PCR amplification of SDIMO alpha-subunit gene
3. RESULTS AND DISCUSSION
3.1.Behavior of pH, DO, MLSS and MLVSS in culture
3.2.Removal of organic matter by MAS process
3.3.Increase in SDIMO gene-carrying bacteria
3.4.Bacterial flora of 1,4-dioxane acclimated sludge
3.5. Usefulness of the MAS process in acclimation and enrichment of degrading bacteria
4. CONCLUSION
ACKNOWLEDGEMENT
REFERENCES