earticle

영어

Although Tricholoma matsutake has been preferred to consumers in Asia, cultivation of these mushrooms has been limited in nature because of symbiotic relationship between T. matsutake and its host, Pinus densiflora. To expand the variety of cultivation methods, co-cultivation with T. matsutake mycelium and fungi from shiro which T. matsutake fruiting body grow was processed in this study. In two Korean provinces, Bonghwa and Yangyang-gun, T. matsutake mycelium and 656 fungal isolates from shiro were collected. Based on the homogeneity of morphological features, 155 fungal isolates were selected for the co-cultivation. From the selected fungal isolates, filtered fungal metabolites were produced and injected on T. matsutake mycelium. As results, metabolites from 9 fungal isolates exhibited promoted growth of T. matsutake mycelium. Sequencing ITS region was processed using two primers (ITS4 and ITS5) and the 9 fungal isolates were identified (3 Penicillium, 2 Aspergillus, 1 Umbelopsis, 1 Penicillago, and 1 Cladosporium). These fungal isolates are expected to contribute on promoted growth of T. matsutake.