earticle

영어

As a one of the most favorite food in Asia and member of ectomycorrhizal mushroom, Tricholoma matsutake has been symbiotic relationship with its host, Pinus densiflora. For artificial cultivation of T. matsutake, several experiments have been introduced like P. densiflora which infected with T. matsutake mycelium. In this study, bacteria from shiro which T. matsutake fruiting body grow was co-cultivated with T. matsutake mycelium to promote the growth of mycelium. From two regions, Bonghwa and Yangyang-gun, T. matsutake mycelium and bacterial strains from shiro were collected. Among the collected bacterial strains, 127 isolates were selected for the co-cultivation based on the homogeneity of morphological features. As results, 13 bacterial isolates exhibited promoted growth of T. matsutake mycelium. In addition, sequencing 16S rRNA region of 13 bacterial isolates was processed using two primers, 27F primer and 1492R primer. As result, 5 genus of bacteria were identified (2 Bacillus, 1 Microbacterium, 6 Paenibacillus, 2 Peribacillus, and 2 Pseudomonas). Result of this study is expected to contribute on promoted growth of T. matsutake.