earticle

영어

This study evaluated the antioxidant activity of the extract from Chaetomorpha torta, and measured its anti-inflammatory and whitening activities. First, the antioxidant activity was evaluated, and the EC50 was found to be 4.68 ± 0.68 mg/mL in the DPPH radical scavenging assay, and 8.99 ± 2.70 mg/mL in the ABTS radical scavenging assay. Total phenolic contents and total flavonoid contents were found to be 9.65 ± 0.64 mg gallic acid/g and 2.93 ± 0.06 mg quercetin/g. Subsequently, a cell experiment using B16F10 was conducted to investigate the whitening activity. In the whitening activity assay, there was no cytotoxicity in the range of 12.5-100 μg/mL, and a melanin synthesis inhibition effect of 32.5 ± 3.3% was observed at 100 μg/mL. To investigate the anti-inflammatory activity, a cell experiment using Raw 264.7 was performed. In the anti-inflammatory activity assay, there was no cytotoxicity in the range of 12.5-100 μg/mL, and 80.3 ± 1.6% inhibition of NO production was observed decreasing proportionally to the extract treatment concentration.

한국어

본 연구는 녹조류인 Chaetomorpha torta 추출물의 항산화능을 평가하고, 이를 바탕으로 항염 활성 측정 과 미백 활성 측정을 진행하였다. 먼저 항산화능의 측정을 진행하여 DPPH 라디칼 소거능 측정에서 EC50은 4.68±0.68 mg/mL로 나타났으며, ABTS 라디칼 소거능 측정에서 EC50은 8.99±2.70 mg/mL로 나타났다. Total phenolic contents와 total flavonoid contents는 각각 9.65±0.64 mg gallic acid/g, 2.93±0.06 mg quercetin/g으 로 나타났다. 그 후 미백 활성을 알아보기 위해 B16F10을 이용한 세포실험을 진행하였다. 미백 활성 측정에서 세 포 독성은 12.5-100 μg/mL의 구간에서 세포독성을 나타내지 않았으며, 100 μg/mL에서 32.5±3.3%의 멜라닌 생성 억제효과를 나타내었다. 항염 활성을 알아보기 위해서 Raw 264.7을 이용한 세포실험을 진행하였다. 항염 활성 측 정에서 세포 독성은 12.5-100 μg/mL의 구간에서 세포독성을 나타내지 않았으며, 100 μg/mL에서 80.3±1.6%의 NO 생성 억제효과를 나타내었다.