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Purpose: We investigated the effects of the hold-relax and foam roller techniques on the range of motion, flexibility, and tone of shortened hamstring muscles. Method: The study included 24 participants who were randomly categorized into hold-relax and foam roller groups. Both groups performed the assigned task thrice a week for a total of 5 sets of 15 s each on the hamstring muscles for 6 weeks. Range of motion was evaluated using a goniometer, flexibility was evaluated using the sit and reach test, and muscle tone was measured using a myotone. We evaluated joint range of motion, flexibility, and muscle tone pre- and 6 weeks post intervention in both groups. Results: We observed a significant intergroup difference in range of motion and flexibility after 6-week training. Conclusion: The hold-relax technique was more effective than the foam roller technique for stretching exercise to improve range of motion and flexibility of shortened hamstring muscles.