원문정보
초록
영어
Brassica oleracea var. italica (broccoli) is a type of cabbage that contains vitamins, minerals, and phytochemicals. Consequently, it is used as a potential nutraceutical source for improving human health by reducing oxidative stress and inflammatory responses. Here, the effects of broccoli sprout extract (BSE) on the inflammatory response were investigated through lipopolysaccharide (LPS)- induced inflammatory mouse models. First, we found that the BSE obviously reduce NO production in RAW 264.7 cells in response to LPS stimulation in in vitro study. Pretreatment with BSE administration improved sperm motility and testicular cell survivability in LPS-induced endotoxemic mice. Additionally, BSE treatment decreased the levels of the pro-inflammatory cytokines TNF-a, IL-1β, and IL-6, and COX-2 in testis of LPS-induced endotoxemic mice models. In conclusion, BSE could be a potential nutraceutical for preventing the excessive immune related infertility.
목차
INTRODUCTION
MATERIALS AND METHODS
Preparation of BSE and HPLC
Cell culture and cell viability assay
Nitrate scavenging ability assay
Animals and treatment
Isolation of RNA and qPCR analysis
Western blot studies
Histology and immunostaining
Sperm motility
Statistical analysis
RESULTS
HPLC analysis of BSE
Effect of BSE on cell viability RAW 364.7 macrophage and NO production
Effects of BSE on LPS-induced inflammation on the testis
BSE suppressed Pro-inflammatory response in testis of LPS-induced inflammation model.
DISCUSSION
CONCLUSION
REFERENCES