earticle

영어

Purpose: This study aimed to investigate the use of purified Kaki-tannin obtained from a Korean natural product, Diospyros kaki Thumb. In order to confirm the potential application of kaki-tannin to cosmetics, the efficacy was evaluated by kaki-tannin that molecular weight decreased by gamma irradiation. Methods: Kaki-tannin polymer was extracted by hydrothermal extraction from persimmon. The molecular weight of Kaki-tannin polymer reduced by gamma-ray irradiation, and then separated and purified. Further, the molecular weight of this polymer was reduced by gamma-ray irradiation; subsequently, it was separated and purified. Radical scavenging activities of this reduced molecular weight Kaki-tannin were examined by the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation, and hydroxyl radical scavenging assays. Moreover, the inhibitory effects of Kaki-tannin on tyrosinase and melanin synthesis in B16F10 melanoma cells were measured in vitro. Furthermore, the expression of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2 during melanogenesis was confirmed by western blotting. Results: Irradiated Kaki-tannin exhibited an antioxidant effect as well as suppressed tyrosinase activity and melanogenesis. The results of this study confirmed that the expression of tyrosinase, MITF, and TRP-2 decreased as the treatment of irradiated Kaki-tannin increased. Conclusion: In the present study, to incorporate high molecular weight Kaki-tannin in cosmetics, it was first irradiated with gamma rays in order to reduce its weight. This study confirmed that Kaki-tannin irradiated by gamma-ray has antioxidant and whitening effects as well as the potential to be used as a cosmetic material.

한국어

목적: 화장품의 기초 소재 개발방안 중 하나로, 국내 천연물인 청도 감을 이용하여 감 탄닌을 추출하였다. 30,000 Da의 큰 분자량 을 가지는 감탄닌을 화장품에 효능 성분으로 이용 가능하도록 방사선 조사를 통하여 저분자화 하여 그 효능을 평가하였다. 방법: 감을 시료로 하여 열수추출을 통하여 고분자 감탄닌을 추출하였으며 고분자 감탄닌에 Gamma ray를 조사하여 저분자화 하고 분 리 정제 하였다. 방사선이 조사된 감탄닌에서 DPPH, ABTS+ 및 Hydroxyl 라디칼 소거능을 측정하였으며, in vitro상의 tyrosinase 활성 저해 효과와 B16F10 melanoma cell에서의 멜라닌 합성 억제 효과를 관찰하였다. 또한 그 때의 멜라닌 합성 관련 단백질 발 현량을 western blotting을 통하여 확인하였다. 결과: In vitro 시험 결과, 방사선이 조사된 감탄닌은 항산화 효과를 나타내었으 며, tyrosinase의 활성과 melanogenesis가 억제 됨을 확인하였다. 또한 방사선이 조사된 감탄난의 시료 농도가 증가함에 따라 Tyrosinase, MITF, TRP-2 발현양이 감소함을 확인하였다. 결론: 본 연구를 통하여 고분자 감탄닌에 방사선을 조사하여 저분자 감 탄닌을 만들고 제조된 저분자 감탄닌이 항산화, 미백 효능을 나타내어 화장품 소재로써 이용될 잠재력이 있음을 확인였하다.

중국어

目的: 本研究旨在研究从韩国天然产品柿蒂（Diospyros kaki Thumb）中提取的纯化柿子单宁在化妆品中的潜 在应用。此外，通过γ射线照射降低其分子量来评估柿子单宁的功效。方法: 首先通过热水提取法提取高分子 柿子单宁聚合物。此外，通过γ射线照射其聚合物的分子量降低，随后，进行分离和纯化。利用对γ射线照射的 Kaki-tannin进行 DPPH、ABTS+ 和羟基自由基清除活性测定。此外，在体外测量了柿子单宁对B16F10黑色素 瘤细胞中酪氨酸酶和黑色素合成的抑制作用。此外，蛋白质印迹法证实了黑素生成过程中酪氨酸酶、小眼相关 转录因子 (MITF)、酪氨酸酶相关蛋白 (TRP)-1和TRP-2的表达。结果: 经辐照的柿单宁表现出抗氧化作用以及抑 制酪氨酸酶活性和黑色素生成。这项研究的结果证实，酪氨酸酶、MITF 和 TRP-2的表达随着辐照柿单宁处理的 增加而降低。结论: 在本研究中，为了在化妆品中加入高分子柿子单宁，首先用γ射线照射以减少其分子量。经 研究发现，照射的柿子单宁具有抗氧化和美白作用，并有潜力用作化妆品原料。