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Purpose: The purpose of this study is to analyze air quality and carbon emissions in developing countries, particularly Myanmar, and explore the impact of transportation on CO2 emissions during peak hours relative to free-flow conditions. Method: This study conducted a traffic survey in two major cities in Myanmar to quantify carbon dioxide emissions from the transportation sector, using IPCC's tier 1 and tier 2 approaches, with statistical analysis performed using Python 3 and Microsoft Excel for comparative analysis of critical factors in CO2 emissions. Result: The result of this study is an estimate of the vehicle kilometers traveled (VKT) and fuel consumption in Yangon city for the year 2019, based on data from various sources including the Myanmar Statistical data base, YUTRA project survey, and Ministry of Electric and Energy. The study also analyzes the average travel time index (TTI) for the four roads in Yangon, which indicates the impact of congestion on vehicle travel time and CO2 emissions. Overall, the study provides important insights into the transport sector in Yangon city and can be used to inform policies aimed at reducing greenhouse gas emissions and improving traffic conditions. Conclusion: The study concludes that congestion plays a significant role in increasing fuel use and emission levels in the road transport sector in Myanmar. The analysis provides valuable insights into the impact of the sector on the environment and emphasizes the importance of addressing congestion to reduce fuel use and emissions. However, the study's scope is limited to Yangon city and Mandalay city, and some mean values may not accurately represent the entire country and other developing countries.