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Background: This study was aimed at investigating the perception of the requirement of measuring the periodontal pocket depth of the dental hygiene students in the mock national board examination. Methods: SPSS 25.0 (IBM SPSS Statistics) was used to perform the independent t-test for the recognition level of dental hygiene students in the interdisciplinary practical national board examination. A p-value < 0.05 was set to indicate statistical significance. Results: Participants showed a high recognition level for the requirement of measuring the periodontal pocket depth without any difference among disciplines. When periodontal pocket depth measurement was included in the national board examination, the educational and clinical work of dental hygienists was expected to be unified, and the professionalism of dental hygienists was expected to be strengthened. Conclusion: The results of this study suggest that the feasibility of introducing periodontal pocket depth measurement in the practical national board examination for dental hygienists should be reviewed by a group of experts.