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Background: This study was aimed at investigating the type of work of dental hygienists and identifying factors affecting the subjective health status according to hazardous risk factors in the working environment. Methods: From May 15 to 31, 2019, we surveyed 411 dental hygienists working at dental hospitals in the metropolitan area. SPSS 25.0 was used to perform the frequency analysis, t-test, one-way analysis of variance, Pearson’s correlation analysis, and multiple regression analysis. Results: Regarding factors related to the degree of exposure to hazardous risk factors in the work environment and subjective health status, the more exposed the ergonomic (p<0.05) and psychological (p< 0.001)factors, the worse is the subjective health status. Conclusion: The results of this study suggest that the working environment should be improved for dental hygienists such that they can perform their duties in a safe environment.