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Purpose : Recently, infection control in childcare centers has increased due to COVID-19. This studyaimedto improve the quality of childcare services. Methods : For this study, childcare facilities were grouped into two groups depending on whether thechildcarecenter employed a nurse as a childcare teacher responsible for the health management of children. The level of COVID-19 infection control job stress was investigated, and the effect of COVID-19 infection control job stress on burnout was also analyzed. The data were collected from 130 childcare teachers with nurses and 184 without nurses in Seoul, Incheon, Gyeonggi, and Daejeon. A questionnaire survey was conducted through mail or the Internet, and this study carried out a multiple linear regression analysis to discover the effects of childcare teachers’ COVID-19 infection control job stress on burnout. Results : The results of this studyaresummarized as follows. First, the scores of the COVID-19 infection control job stress and burnout were higher for childcare teachers without nurses than those with nurses. Second, for those childcare teachers with nurses, their COVID-19 Infection control stress did not affect their burnout, but for childcare teachers without nurses, the quantitative workload among the sub-factors of COVID-19 infection control stress did affect burnout. Conclusion : Accordingly, it is necessary to allow co-nurses of several facilities to be grouped based on the capacity ofchildcarecenters with 100 children.