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Purpose: To compare respiratory muscle function on the paretic and non-paretic sides in patients diagnosed with stroke. Methods: Forty-four patients clinically diagnosed with ischemic or hemorrhagic stroke were included. The diaphragm and parasternal intercostal muscles were measured for muscle thickness and movement, respectively. Respiratory muscle activity was measured in the diaphragm, external intercostal muscles, and sternocleidomastoid muscles. The measurement data were analyzed using an independent t-test to compare the paretic and nonparetic sides of respiratory muscle function. Results: The diaphragm thickness at total lung capacity, thickness ratio, and parasternal intercostal muscle movement of the non-paretic side were significantly greater than those of the paretic side. Moreover, the diaphragm activity of the paretic side was significantly greater than that of the nonparetic side. Conclusion: TThis study demonstrated that asymmetry of respiratory muscle function between the paretic and non-paretic sides occurred after stroke. To improve the respiratory function of stroke patients, respiratory muscle strengthening exercises are needed to improve the asymmetry of the respiratory muscle function.