원문정보
Protective Effect of Placental Extract against Oxidative Stress through Autophagy Activity in Schwann Cells
초록
영어
Schwann cells play a critical role for myelination in peripheral nerve system. It also plays an important role in nerve protection and regeneration. In peripheral nerve damage, regeneration is induced by the migration and proliferation of Schwann cells which were promoted by suppressing the oxidative stress. In this study, Human placental extract was prepared by homogenization and estimated its efficacy in RSC96 cells. Placental extract exhibited a protective effect against hydrogen peroxide-induced oxidative stress in RSC96 cells, confirmed by MTT assay. Furthermore, placental extract decreased intracellular ROS against oxidative stress, confirmed by DCFH-DA assay. Autophagy was visualized with Cyto-ID staining to confirm the autophagy activity of placental extracts. The activity of autophagy was confirmed by immunoblot analysis of autophagy flux-associated proteins such as LC3 conversion and SQSTM1 degradation. Thus, we confirmed the antioxidant effect of placental extract to protect RSC96 cells from oxidative stress, and observed that it activated autophagy and restored autophagy flux.
목차
1. Introduction
2. Materials and Methods
2.1 세포 배양
2.2 세포 생존률 분석
2.3 세포내 ROS 검출
2.4 자가포식 검출 분석
2.5 면역블롯 분석
2.6 통계 분석
3. Results and Discussion
3.1 태반 추출물의 산화스트레스에 대한 보호효과
3.2 태반 추출물의 세포내 ROS의 제거
3.3 태반 추출물의 자가포식 활성
3.4 태반 추출물의 자가포식 플럭스의 회복 능력
4. Conclusion
References