원문정보
초록
영어
In this study, the effect of chronic alcohol intake for 4 weeks on the muscular atrophy factors of rat skeletal muscle was studied using 6-week-old growing Sprague-Dawley rats. Experimental animals were classified into a control group and an alcohol intake group. The alcohol intake group consumed alcohol orally at a concentration of 3-g/kg body weight every day for 4 weeks. The control group consumed tap water in the same way. After 4 weeks alcohol ingestion, glucose, total choles-terol, triglyceride, and high-density lipoprotein cholesterol in serum lev-els were measured. Western blot was performed to detect the expres-sions of muscle RING-finger protein-1 (MuRF1), protein kinase B (Akt), phosphorylated Akt (p-Akt), forkhead box O (FoxO), phosphorylated FoxO (p-FoxO), p38, and phosphorylated p38 (p-p38). Results of this experiment showed that chronic alcohol intake enhanced triglyceride concentra-tion. Chronic alcohol intake increased MuRF1 expression to promote muscle proteolysis and decreased p-Akt/Akt ratio and p-FoxO/FoxO ra-tio to inhibit skeletal muscle growth. Therefore, alcohol consumption has been shown to cause muscle atrophy.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals and treatments
Blood sampling and serum component analysis
Muscle tissue pretreatment
Western blotting
Data analysis
RESULTS
Effect of alcohol intake on blood composition
Effect of alcohol intake on MuRF1 expression
Effect of alcohol intake on ratio of p-Akt/Akt expression
Effect of alcohol consumption on ratio of p-FoxO/FoxO expression
Effect of alcohol consumption on ratio of p-p38/p38 expression
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES