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Background/Objectives: The purpose of this study was to review the molecular diagnosis of coronavirus disease as COVID-19 was a worldwide pandemic from 2019. In addition, by providing accurate information on the molecular diagnostic tools currently in use, it was intended to be helpful in determining the timing of treatment and in public health policies. Methods/Statistical analysis: In this study, to examine the use of diagnostic tools related to COVID-19, “Omicron”, “Diagnosis”, “RT-PCR”, “COVID-19”, and “Analysis” were searched as keywords through Google and the literature on the latest diagnostic techniques was used. Findings: Recently, it was announced that the fatality rate of Omicron, which has a high epidemic rate and high infection rate, was 1/4 of that of Delta. SARS-CoV-2 Omicron variant RT-PCR laboratory developed assay was said to be sensitive and specific for detecting Omicrons in nasopharyngeal and nasal swabs. The closed tube Penn-RAMP can be used with minimal equipment and training. Improvements/Applications: The purpose of this study was to investigate the types and characteristics of accurate molecular diagnostic tests. These molecular diagnostic tests are considered to be important for prompt and accurate diagnosis, distinguishing infected persons, and coping with appropriate isolation and treatment. In addition, this study was intended to help the general public understand molecular diagnostic testing methods.