earticle

영어

Malignant melanoma, the out-of-growth of abnormal cells in the epidermis, is one of the most aggressive and invasive form of skin cancers which threatens human life expectancy in the world. A ganglioside, a molecule composed of a glycosphingolipid, has crucial roles involving in cell-cell interaction, cell growth, proliferation, inflammation, differentiation, and tumorigenesis. Meanwhile, 7,8-Dihydroxyflavone (7,8-DHF), also well known as tropoflavin and a small flavonoid, is natural polyphenolic compounds found in several vegetables, fruits, and tree leaves. Although 7,8-DHF is considered that potentially inhibited cancer growth, proliferation, invasion, and metastasis, the relationship between its effects on malignant melanoma and ganglioside expression is not fully understand yet. Accordingly, this study was to investigate the anti-cancer effects of 7,8-DHF based on the relationship between protein-protein interaction and ganglioside on human skin cancer. After treatment with 7,8-DHF, we found that abnormal cell elongation and morphological change in the proliferation and growth on melanoma cancer cells. 7,8-DHF directly induced nucleus fragmentation and changed the level of pro- and anti-apoptotic proteins. In our study, we confirmed the mitochondrial membrane permeabilization and mitochondrial ROS production. Also, we found that 7,8-DHF downregulated the expression level of ganglioside GD3 by high performance thin-layer chromatography analysis. This phytochemical was found to be a physiologically active substance that plays an important role in melanogenesis and has anti-cancer efficacy by inhibiting the expression and activity of ganglioside. Taken together, it might be an important evidence for treating malignant melanoma to figure out the molecular mechanisms.