earticle

영어

Saccharapolyspora spinosa is the producer of the environmentally friendly insecticide spinosad, which is a combination of spinosyn A and D. spinosyn is a tetracyclic polyketide aglycone to which is attached a neutral saccharide substituent (2,3,4-O-trimethyla-L-rhamnosyl) on the C-9 hydroxyl group and an aminosugar moiety (β D-forosaminyl) on the C-17 hydroxyl group). Both the rhamnose and forosamine moieties are essential for the insecticidal activity of spinosyns. We construct a vector; pIBR-SPN, cloned the sugar genes to it and transformed to Sa. spinosa. The best strain Sa. spinosa MUV pIBR-SPN FR produced 1,896.77 mg/L of spinosad, 17 folds higher than parent strain.