earticle

영어

A highly efficient biocatalytic system was constructed to make bioactive resveratrol poly-glucosides in a chain by using sucrose as a cheap source of UDP-glucose donors with the help of amylosucrase, DgAS from Deinococcus geothermalis. The system is superior as the reaction is very fast, direct, high rate of conversion of substrates to products, high thermostable, and regio-stereospecific with very effective in action at 40°C. Successfully our system can convert around 98% resveratrol to its resveratrol glucosides in a short time. The amylosucrase DgAS not only added glucose in multiple hydroxyl positions but also conjugates glucose in the chain as a result highly soluble glucosylated products are formed. Because of the highest thermostability and specific action among all known amylosucrases (ASases), DgAS has proven its importance for the transglucosylation activities in different polyphenols on an industrial scale. Therefore, our system can be used industrially for the cost-effective production of resveratrol glucosides.