원문정보
초록
영어
EpCAM is a tumor-related antigen and is a cell surface glycoprotein expressed in colon cancer. EpCAM has been considered a potential target for tumor vaccines. In this study, Agrobacterium-mediated transformation was applied to produce genetically modified Chinese cabbage plants to express EpCAM-IgM FcK. To avoid the plant-specific N-glycosylation process occurring in Golgi devices, the KDEL ER sequence was attached to the C-terminal of Fc to refair the glycoprotein of ER. The recombianat protein productivity was maximized by developing a safe and efficient plant cell factory system using a genetically modified plant cell line expressing EpCAM-IgM FcK protein. In addition, plant seedlings expressing both EpCAM-IgM FcK and J-chain K were obtained by crossing. PCR confirmed that the EpCAM-IgM FcK gene present in the seedlings. Western Blot screened seedlings with high expression. Chinese cabbage protoplasts were obtained by successfully optimizing the protoplast separation protocol to perform single cell transcription analysis. Single cell transcription analysis was performed to reveal the cell types that highly express EpCAM-IgM FcK protein. Cell lines with high expression will be applied to plant cell factory systems to produce EpCAM-IgM FcK.