earticle

영어

3D bioprinting is the technique to precisely control 3D tissue constructs, combining with cells, growth factors, and/or other biomolecules. It provides tissue-like 3D complexity, which meets critical needs in drug discovery, cosmetics testing, medical research, and artificial organ replacement by mimicking anatomical shapes and environments. Nevertheless, there have been only a few examples of protein-based bioinks due to its poor printability. Here the silk protein was blended with carrageenan to resolve this restrictions. Carrageenan, a sulphated linear polysaccharide from marine, has shown the temperature - derived reversible sol/gel transition. Since its viscosity varies readily by temperature, its rheological properties are manipulatable to satisfy the properties of a printable bioink. The interaction between silk protein and carageenan is physically induced, and the interaction within silk is generated by photo-crosslinking reaction. The bioink presented suitable viscosity and fast sol/gel transition to stack tens of layers, as well as durable modulus which is similar to skin. The sulfate chains of carrageenan was also advantageous to recruit cell binding, and exhibited improved cell viability.