원문정보
초록
영어
Understanding glycan-protein interactions provide valuable information on biological processes in living organisms. However, many roles of glycans in biology are still veiled. The primary reason might be the limitation of access to a glycan library with diverse structures, which hampers extensive studies on molecular interactions of glycans. Here, a functional glycan chip combined with on-chip enzymatic glycosylation was developed to prepare complex glycan sources and apply glycan-involved applications simultaneously. GM1 pentasaccharide and Globo H hexasaccharide-related complex glycans were successfully synthesized on a chip through consecutive glycosyltransferase reactions along with small amounts of enzymes and donors, without any additional processes. Biosynthesized complex glycans were demonstrated to provide information on glycan-related interactions. Thus, the proposed on-chip glycan biosynthesis system can provide a new direction toward obtaining complex glycan sources and complex glycan-involved applications such as glycan-protein interaction analysis and glycan biomarker screening.