earticle

영어

High-throughput single-cell RNA sequencing (scRNA-seq) technology provide opportunities to identify the entire cellular and molecular difference based on cell-to-cell heterogeneity in gene expression. This study demonstrates the feasibility and utility of scRNA-seq in transgenic Nicotiana tabacum (N. Tabacum) leaves expressing anti-rabies virus mAb(P) SO57 and provides generation gene expression map of the plant leaf at single-cell resolution. Protoplasts isolated from the N. Tabacum leaves at non-transgenic and transgenic plants, and the transcript levels of > 20,000 genes were analyzed in 24,000 single cells from non-transgenic and transgenic plants. Total of 9 putative cell clusters and the cluster-specific marker genes were identified. We performed cell clustering analysis to determine which cell clusters were mainly synthesis exogenous genes. In conclusion, the high-resolution single cell atlas of gene expression shows the developmental landscape of N. tabacum leaf and exogenous gene expression patterns in each clusters.