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Purpose: This study aims to examine the effect on the lower extremity muscle activity of soccer player having chronic ankle instability by mediating neuromuscular training combining transcranial direct current stimulation. Method: Thirty college soccer players were selected as the participants of study, and divided into a neuromuscular training group combining transcranial direct current stimulation (Experimental group) and a general neuromuscular training group (Control group), and 15 participants randomly assigned. The participants of this study additionally intervented neuromuscular training combining transcranial direct current stimulation and general neuromuscular training to each group for 30 minutes five times a week for eight weeks after receiving general soccer training. The attached to active C3, C4, CZ. Lower extremity muscle activity was analyzed before the intervention. Tibialis anterior, gastrocnemius and soleus were measured by maximum voluntary isometric contraction in the in the bare-handed muscle strength test posture in order to analyze the lower extremity muscle activity. Above outcomes were equally remeasured and in intergroup analysis was conducted after eight weeks of intervention. Result: The neuromuscular training group combining transcranial direct current stimulation (Experimental group) showed a statistically significant difference in lower extremity muscle activity compared to the general neuromuscular training group (Control group) as a result of comparing and analyzing the lower extremity muscle activity between two groups(p<.05). Conclusion: It was found that the neuromuscular training combining transcranial direct current stimulation is more effective in improving lower extremity muscle activity as a result of above. The basic data on the possibility of using tDCS in the filed of soccer player sports rehabilitation could be provided based on this study.