원문정보
초록
영어
The acrosome cap allows sperm to penetrate the egg membrane and produce male pronuclei within female chicken eggs, facilitating successful fertilization. Given this, it is important to establish practical methods for evaluating the integrity of the acrosome cap and thus the quality of the rooster’s sperm. There are several established methods for evaluating the acrosomes of mammalian sperm, but none of these methods are suitable for evaluating the acrosome status of rooster spermatozoa. Therefore, a simplified method for evaluating the rooster acrosome is needed. Here we evaluated the usefulness of CBB (coomassie brilliant blue) staining of the acrosome at concentrations of 0.04%, 0.08%, and 0.3% CBB solutions. Our data revealed a clear staining pattern for intact acrosome caps at 0.04% and 0.08% CBB but not at 0.3% CBB. This protocol revealed differences in acrosome integrity between fresh and frozen rooster sperm smears suggesting that CBB staining may facilitate easier semen evaluation in roosters. This protocol allows for the accurate differential staining of acrosome cap in rooster spermatozoa.
목차
INTRODUCTION
MATERIALS AND METHODS
Animals and ethics statement
Chemicals and reagents
Semen treatment for fresh, heat-stressed and cryo-damaged spermatozoa
Staining procedures and microscope examination of semen smear
Acrosome integrity analysis
Statistical analysis
RESULTS
Morphological examination of acrosome region by Diff Quik stains
Effects of CBB concentration on differential staining between head region and acrosome cap
The rate of acrosome cap intactness in fresh, heat-stressed, and cryopreserved semen
DISCUSSION
CONCLUSION
REFERENCES