원문정보
초록
영어
Skin is the largest organ of humans. Overexposure to ultraviolet (UV) is the primary environmental factor that causes skin damage. The compound, (-)-loliode, isolated from the brown seaweed Sargassum horneri, showed strong antioxidant and anti-inflammatory activities in in vitro and in vivo models. To further explore the potential of (-)-loliode in cosmetics, in the present study, we investigated the photoprotective effect of (-)-loliode in vitro in skin cells and in vivo in zebrafish. The results indicated that (-)-loliode significantly reduced intracellular reactive oxygen species (ROS) level, improved cell viability, and suppressed apoptosis of UVB-irradiated human keratinocytes. In addition, (-)-loliode remarkably attenuated oxidative damage, improved collagen synthesis, and inhibited matrix metalloproteinases expression in UVB-irradiated human dermal fibroblasts. Furthermore, the in vivo test demonstrated that (-)-loliode effectively and dose-dependently suppressed UVB-induced zebrafish damage displayed in decreasing the levels of ROS, nitric oxide, lipid peroxidation, and cell death in UVB-irradiated zebrafish. These results indicate that (-)-loliode possesses strong photoprotective activities and suggest (-)-loliode may an ideal ingredient in the pharmaceutical and cosmeceutical industries.
목차
1. Introduction
2. Results and Discussion
2.1. Protective Effect of (-)-Loliode against UVB-Induced HaCaT Cell Damage
2.2. Protective Effect of (-)-Loliode against UVB-induced HDF Cell Damage
2.3. Protective Effect of (-)-Loliode against UVB-induced Zebrafish Damage
3. Materials and Methods
3.1. Chemical and Regents
3.2. Preparation of (-)-Loliode from S. horneri
3.3. In Vitro in HaCaT cells
3.4. In Vitro in HDF cells
3.5. In Vivo Assay
3.6. Statistical Analysis
4. Conclusions
References