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Molecular characterization and immune regulatory, antioxidant, and antiapoptotic activities of thioredoxin domain-containing protein 17 (TXNDC17) in yellowtail clownfish (Amphiprion clarkii)

초록

영어

Thioredoxin domain-containing protein 17 (TXNDC17) is an important, highly conserved oxidoreductase protein, ubiquitously expressed in all living organisms. It is a small (~14 kDa) protein mostly co-expressed with thioredoxin 1 (TRx1). In the present study, we obtained the TXNDC17 gene sequence from a previously constructed yellowtail clownfish (Amphiprion clarkii) (AcTXNDC17) database and studied its phylogeny as well as the protein’s molecular characteristics, antioxidant, and antiapoptotic effects. The full length of the AcTXNDC17 cDNA sequence was 862 bp with a 372 bp region encoding a 123 amino acid (aa) protein. The predicted molecular mass and isoelectric point of AcTXNDC17 were 14.2 kDa and 5.75, respectively. AcTXNDC17 contained a TRX-related protein 14 domain and a highly conserved N-terminal Cys43-Pro44-Asp45-Cys46 motif. qPCR analysis revealed that AcTXNDC17 transcripts were ubiquitously and differently expressed in all the examined tissues. AcTXNDC17 expression in the spleen tissue was significantly upregulated in a time-dependent manner upon stimulation with lipopolysaccharide (LPS), polyinosinic-polycytidylic (poly I:C), and Vibrio harveyi. Besides, LPSinduced intrinsic apoptotic pathway (TNF-α, caspase-8, Bid, cytochrome C, caspase-9, and caspase-3) gene expression was significantly lower in AcTXNDC17-overexpressing RAW264.7 cells, as were NF-κB activation and nitric oxide (NO) production. Furthermore, the viability of H2O2-stimulated macrophages was significantly improved under AcTXNDC17 overexpression. Collectively, our findings indicate that AcTXNDC17 is involved in the innate immune response of the yellowtail clownfish.

목차

ABSTRACT
1. Introduction
2. Materials and methods
2.1. Experimental animals and tissue sampling
2.2. Total RNA extraction and cDNA synthesis
2.3. cDNA database construction
2.4. Sequence identification and molecular characterization of AcTXNDC17
2.5. AcTXNDC17 expression analysis
2.6. Cloning of AcTXNDC17 into expression plasmids
2.7. Expression and purification of the AcTXNDC17 protein
2.8. Functional studies
3. Results
3.1. In silico analysis of AcTXNDC17
3.2. Tissue-specific distribution of AcTXNDC17
3.3. AcTXNDC17 expression in response to immune stimuli
3.4. Expression and purification of rAcTXNDC17 protein
3.5. Functional activity
4. Discussion
5. Conclusion
References

저자정보

  • H.M.V. Udayantha Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • Anushka Vidurangi Samaraweera Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • Kishanthini Nadarajapillai Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • W.M.Gayashani Sandamalika Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • Chaehyeon Lim Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • Hyerim Yang Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, South Korea
  • Sukkyoung Lee Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, South Korea
  • Jehee Lee Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, South Korea

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