원문정보
초록
영어
Overexposure to ultraviolet B (UVB) causes skin damage. The purpose of this studywas to evaluate the protective effect of a fucoidan with a molecular weight of 102.67 kDa, isolated from Hizikia fusiforme, against UVB-induced photodamage in vitro in human dermal fibroblasts (HDFs) and in vivo in zebrafish. Fucoidan remarkably inhibited commercial collagenase. Additionally, it significantly and dose-dependently decreased the intracellular reactive oxygen species (ROS) levels and increased the viability of UVB-irradiated HDFs. Furthermore, fucoidan remarkably improved collagen synthesis, inhibited intracellular collagenase, and reduced the expression of matrix metalloproteinases and pro-inflammatory cytokines in UVB-irradiated HDFs. Further research demonstrated that these effects occurred through the regulation of the activator protein 1, nuclear factor kappa B, and mitogenactivated protein kinase signaling pathways. Furthermore, the in vivo results showed that fucoidan protected zebrafish larvae against UVB-induced photodamage by decreasing cell death via the suppression of lipid peroxidation and inflammatory response through ROS clearance. In conclusion, fucoidan isolated fromHizikia fusiforme exhibits strong in vitro and in vivo photoprotective effects, and can be used as an ingredient in the cosmeceutical and pharmaceutical industries.
목차
1. Introduction
2. Materials and methods
2.1. Reagents and chemicals
2.2. Preparation of fucoidan and determination of its effect on collagenase
2.3. Cell culture and UVB irradiation
2.4. Determination of effect of fucoidan on UVB-induced damage to HDFs
2.5. Determination of relative intracellular collagenase activity of UVB-irradiatedHDFs
2.6. Determination of collagen,MMP, PGE2, and pro-inflammatory cytokine levels in UVB-irradiated HDFs
2.7. Western blot analysis
2.8. Application of fucoidan and UVB to zebrafish
2.9. Statistical analysis
3. Results and discussion
3.1. Fucoidan inhibits commercial collagenase
3.2. In vitro protective effect of fucoidan against UVB-induced dermic damagein HDFs
3.3. Fucoidan inhibits MMP, PGE2, and pro-inflammatory cytokine expressionin UVB-irradiated HDFs
3.4. Fucoidan regulates NF-κB, AP-1, and MAPK pathways in UVB-irradiatedHDFs
3.5. In vivo protective effect of fucoidan against UVB-induced photodamage in zebrafish larvae
4. Conclusion
References