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Expression and function analysis of anti-HER2 positive breast cancer recombinant mAb and VHH in transgenic plant

원문정보

Se Ra Park, Jeong-Hwan Lee, Sohee Lim, Taek Min Kim, Seung Ho Lee, Dae Heon Kim, Chao Gao, Richard D. Cummings, Kisung Ko

한국당과학회 한국당과학회 학술대회 2021 한국당과학회 연례학술대회 2021.07 pp.78-78
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초록

영어

Overexpression of human epidermal growth factor receptor type 2 (HER2) protein is one of the prognostic factors for breast cancer, and HER2 is most likely to be recurrence when cancer metastasizes to lymph nodes. This study demonstrated the expression of the anti-HER2 camelid single domain antibody VHH in tobacco plant. And we confirmed the function of recombinant VHH for the treatment of breast cancer. The anti-HER2 VHH was fused to human IgG Fc domain along with KDEL endoplasmic reticulum (ER) (VHH-FcK) in a gene expression cassette. The plant gene expression cassette was transferred to tobacco plants using Agrobacterium-mediated transformation. The transformants were screened by PCR and western blots. ELISA confirmed the binding activity of purified anti-HER2 VHH-FcK to HER2 positive breast cancer cell line SK-BR-3. In conclusion, this study verified the possibility of anti-HER2 VHH-FcK as a therapeutic agent and suggests that the anti-breast cancer anti-HER2 VHH-FcK can be expressed, properly assembled and purified from plant expression system, which can be as an alternative system for antibody production. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT)(2021R1F1A1063869).

키워드

저자정보

  • Se Ra Park Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Seoul, Korea
  • Jeong-Hwan Lee Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Seoul, Korea
  • Sohee Lim Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Seoul, Korea
  • Taek Min Kim Major of Nano-Bioengineering, College of Life Sciences and Bioengineering, Incheon-National University, Incheon, Korea
  • Seung Ho Lee Major of Nano-Bioengineering, College of Life Sciences and Bioengineering, Incheon-National University, Incheon, Korea
  • Dae Heon Kim Department of Biology, Sunchon National University, Sunchon, Republic of Korea
  • Chao Gao Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
  • Richard D. Cummings Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
  • Kisung Ko Department of Medicine, Medical Research Institute, College of Medicine, Chung-Ang University, Seoul, Korea

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