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Background/Objectives: The purpose of this study was to examine changes in obesity indicator and blood lipids by participating in weight training after maintaining a fasting for 12 hours in obese middle-aged women for 8 weeks. Methods/Statistical analysis: 8-week exercise program was conducted by dividing into 10 people in the fasting weight training group(WG) and 10 people in the control group(CG). PASW 18.0 Statistical programs were used to present the descriptive statistics of the measurement variables. Comparisons of the two groups were analyzed by 2-way ANOVA. Findings: There was a significant difference between the WG and the CG in TG, TC, HDL and leptin(p<05), but there was no significant difference in irisin(p>.05). Improvements/Applications: The purpose of this study was to examine the effect of fasting exercise on obese middle-aged women. Through this exercise, significant results were obtained in obesity indicator and blood lipid. If future research is conducted through changes in exercise duration and fasting time, meaningful results will be provided.