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Background/Objectives: This study aims to demonstrate pro-inflammatory cytokine production inhibitory effects using Burdock extract. In addition, the basic mechanism of the anti-inflammatory mechanism of burdock is to be studied Methods/Statistical analysis: Thus, This paper was carried out to see the production of NO and pro-inflammatory cytokine ,TNF-α, IL-6 and IL-8 using the macrophages of LPS-treated mice. The cytotoxicity test was used 96 AQueous One solution cell proliferation assay. NO was measured using NO Detection Kit, and the production of pro-inflammatory cytokine was measured using ELISA kit. Findings: As a result, Burdock extract had no cytotoxicity at 10ug/mL to 1,000 ug/mL and significantly inhibited the production of TNF-α as well as IL-6 and IL-8 which are pro-inflammatory cytokines. In the RAW 264.7 macrophage cells, Pro-inflammatory cytokine production inhibitory effects are likely to be diversely utilized as basic physiological activity data and functional materials to demonstrate anti-inflammatory properties of broccoli extract. Improvements/Applications: In conclusion, this study can be used to a basic data to objectively demonstrate the physiological activity of immunological mechanism associated with the anti-inflammatory action of Burdock extract. However, in-depth research on anti-inflammatory is needed.