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Purpose : This study was conducted to investigate the effects of hospital organizational cultures and job satisfaction of male nurses and to identify the relations between these two factors. Mathod : 110 males were involved in the study who worked at 7 university hospitals in Seoul and 2 university hospitals in Gyeonggi area. This survey-based study was analyzed using the SPSS 17.0 statistical program to carry out frequency analysis, t-test, ANOVA, pearson's correlation and multiple linear regression analysis. Results : The mean score of hospital organizational culture was 3.13 points out of 5 points and the mean score of job satisfaction as 2.92 points. the factors that gave significant impacts on job satisfaction were age, education and higher job satisfaction in high, and have faith in the job satisfaction significantly affected. Task-centered organization culture showed appeared to have significant effects on job satisfaction. Conclusion : It is expected to develop programs which can encourage male nurses to fulfill their own goals and to search the way can change the roles and awareness of male nurses and can understand diverse hospital organizations and their organizational cultures.