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Purpose: Low back pain, a common musculoskeletal disorder, is impacted by shortening of the hamstring. Lumbar stabilization exercises are frequently used to treat low back pain. Therefore, this study aimed to investigate the effects of hip abduction angle on medial and lateral hamstring muscle activity during bridge exercise and pelvic posterior tilting exercise. Methods: Thirty-six individuals with herniated intervertebral disk (HIVD) participated in this study. All subjects performed the bridge exercise and pelvic posterior tilting exercise for twelve seconds. While the participants maintained these exercise positions, hamstring muscle activation was measured for each leg. Specifically, dynamic surface electromyography was utilized to compare muscle activity for the biceps femoris and the semitendinosus muscle groups in both affected and non-affected hamstrings. An independent t-test was used to identify the impact of exercise type or symptom presentation on hamstring muscle activity. The data was analyzed using PASW Statistics 18.0 (SPSS Inc, USA). Results: Overall, our results indicated that there was no significant difference in hamstring muscle activation as a consequence of exercise type (p>0.05). This result was observed regardless of the hip joint abduction angle (0° and 30°). However, when participants conducted the bridge exercise while in the hip joint 0° abduction position, muscle activation of both the biceps femoris and the semitendinosus was significantly lower on the affected side than that on the non-affected side (p<0.05). Conclusion: Consequently, performing bridge exercise and pelvic posterior tilting exercises with abducted hip joints may be helpful in the balanced development of the hamstring.