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Purpose: The purpose of this study is to investigate the cardiopulmonary endurance, the energy metabolism and of breathing function in male smoker and nonsmoker in college after graded load exercise using lower cycle ergometer and respiratory measurement device. Methods: For 38 smoker male college students and nonsmoker male college students, an graded load exercise was performed with the use of lower cycle ergometer by increasing 20 watts every 2 minute using ERS 2.0 system and respiratory measurement device for the cardiopulmonary function, energy metabolism and breathing function. Results: Smoker group showed significant differences in maximum oxygen intake, ventilation per minute, and heart rate in cardiopulmonary endurance, and significant differences in body metabolic rate, maximum energy consumption, and anaerobic threshold in energy metabolism. For respiratory function, there was a difference between inhalation pressure and inhalation speed while there was no difference in respiratory function Conclusion: The results showed that smoking negatively would affect aerobic fitness factors including cardiopulmonary endurance and energy metabolism and long-term smoking is expected to aggravate functional factors in male smoking students in the future.