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Purpose: This study investigates the effect of repeated use of lithium disilicate glass-ceramics on color stability. Methods: In this study, 2 types of lithium disilicate glass-ceramics were used and they were divided into 4 groups after 1st, 2nd, 3rd, and 4th heat pressing. The L*, a*, and b* values of all specimens were measured using the VITA Easyshade Advance 4.0. These values was used to determine the ΔE value and transparency. IBM SPSS Statistics ver. 24.0, a statistical significance level of 0.05 and one-way ANOVA were used for all data. Results: In all groups, the L* and b* values were the lowest in the specimens subjected to the 4th heat pressing and the corresponding a* value was the highest. All specimens showed ΔE values of ≤3.3, indicating color changes that were not visible with the naked eye, and the color difference increased with the number of repeated heat pressing. The transparency of all specimens decreased as the number of repeated uses increased. Conclusion: According to the number of repeated heat pressing, the color difference of Rosetta SP (HASSBIO) was larger than that of IPS e.max Press (Ivoclar Vivadent), but it was clinically acceptable in all groups. Moreover, transparency decreased as the number of heat presses increased in all groups. According to the above findings, the lithium disilicate glass-ceramic has high color stability due to repeated heat pressing, implying that it can be used in clinical settings regularly.