earticle

영어

The purpose of this study is to measure the yield and to evaluate the physiological activity of Cannabis sativa seed(Hemp seed) extracts extracted using a density fluctuation supercritical carbon dioxide for each temperature condition-30℃(HSSE30), 45℃(HSSE45), 60℃(HSSE60), and to enable dissolution of the poorly water-soluble extracts by liposome formulation and to enhance the skin permeability. As a result of the yield measurement, HSSE60 showed the highest yield, and in the antioxidant activities, HSSE45 had the highest total polyphenol content, and showed the highest DPPH, ABTS+ radical scavenging activities at the highest concentration of the extracts. As a result of the antimicrobial susceptibility testing, a clear zone appeared only in the Propionibateium acnes strain. It was confirmed that particle size was reduced and the absolute value of the zeta potential increased in the case of the formulation in which the extracts were in liposomes than in the formulation in which the extracts were dissolved in deionized water, and the skin permeability was improved. Based on these experimental results, we confirmed the possibility of using the hemp seed supercritical carbon dioxide extracts, a poorly water-soluble extract, can be applied as a functional natural material for cosmetics.

한국어

본 연구의 목적은 온도조건 별로 밀도요동 초임계 유체를 이용하여 추출한 대마종자 추출물의 수율 측정과 생 리활성능을 평가하고, 리포좀 제형으로 난용성 추출물의 용해를 가능하게 하여 경피투과도를 증진시키는 데 있다. 수 율 측정 결과, 60℃로 추출한 대마종자가 가장 높게 나타났고, 항산화 실험에서 total polyphenol content assay, DPPH radical scavenging assay, ABTS+ radical scavenging assay 결과, 최고농도에서 45℃ 추출물의 폴리페놀 함량과 radical 소거능이 가장 크게 나타났다. 항균실험 결과, Propionibacteium acnes 균주에서만 clear zone이 나 타났다. 추출물을 3차 정제수에 용해시킨 제형보다 리포좀으로 캡슐화한 제형에서 particle size의 감소와 zeta potential의 절댓값 증가를 확인하여 제형 안정성을 확인하였고, 경피투과도의 증진 또한 확인하였다. 이러한 실험결 과를 바탕으로 난용성인 초임계 대마종자 추출물을 화장품의 기능성 천연물 소재로써 활용 가능성을 확인하였다.