원문정보
초록
영어
Many DNA samples are exposed to various environments at crime scenes and mixed with other substances that adversely affect STR profiling. In this study, DNA quantification and STR profiling were performed up to 40 weeks with body wash or hand sanitizer mixed with saliva, blood, and semen at a final concentration of 1%. In addition, serological preliminary tests such as SALIgAE, LMG, and AP and immunological confirmatory tests using RSID kits were conducted to examine the effect of body wash and hand sanitizer on three body fluids. Body wash containing surfactants showed more serious effect on DNA quantity and STR quality than hand sanitizer containing ethanol. Saliva was immediately decomposed after mixing hygiene products, making it impossible to analyze STR, however blood and semen produced full STR profiles after 40 weeks of mixing. As a result, saliva was very vulnerable to hygiene products, but blood and semen were not affected until 40 weeks after mixing. In addition, the discrepancy between serological test and STR profiling in blood and semen samples showed that STR analysis should be performed even if the serological test was negative.
목차
Ⅰ. 서론
Ⅱ. 재료 및 방법
1. 시료채취
2. 위생용품과 체액 시료 혼합
3. 체액 확인 시험
4. DNA 정제 및 정량, STR 프로필 분석
5. 통계처리
Ⅲ. 결과
1. 체액 확인 시험 결과
2. 위생용품별 체액 DNA 정량 결과
3. 위생용품별 체액의 STR 프로필 분석
Ⅳ. 고찰
Ⅴ. 사사
Ⅵ. 참고문헌