원문정보
초록
영어
Purpose: In this study, the neuroprotective effect of the methanol extract of Daphne genkwa Flos (DGF-ex) against glutamate-induced neurotoxicity was studied in mouse hippocampal HT22 cells. Materials and Methods: We conducted the cytoprotective effect of DGF-ex against a 24-hour-stress-induced condition by glutamate in HT22 cells. The inhibitory effect of DGF-ex on cellular necrosis and apoptosis and ROS (reactive oxygen species) was observed using fluorescence microscopy. To further clarify its antioxidant efficacy, assays on SOD (superoxide dismutase) regulation and DPPH/ABTS radical scavenging ability were also conducted. A chemical profile of DGF-ex was also obtained via UPLC analysis. Results: Pretreatment with DGF-ex significantly reduced glutamate-induced loss of cell viability and release of LDH (lactate dehydrogenase). DGF-ex showed similar effects to the antioxidant quercetin (15μM) in the evaluation of DPPH/ABST radical scavenging activity and SOD activity evaluation. In addition, DGF-ex remarkedly downregulated the glutamate-induced programmed HT22 cell death, which was shown in the normalization of the cellular necrosis and apoptosis ratio. Conclusion: These results exhibit DGF-ex has the ability to protect against glutamate-induced cytotoxicity in the HT22 cell line via the drug candidate’s antioxidant capacity. This research confirmed the DGF-ex as a valuable drug candidate for the prevention and treatment of oxidative stress-mediated neurodegenerative diseases.
목차
서론
본론
1. 실험재료 및 방법
2. 실험결과 및 고찰
결론
감사의 글
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