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Original Article

글루타메이트에 의한 산화적 스트레스를 받은 HT22 세포주에서 원화 메탄올 추출물의 신경세포 보호 활성

원문정보

Neuroprotective Effect of Methanol Extration of Daphne genkwa Flos against Glutamate-induced Oxidative Stress in HT22 Cells

노경옥, 김경한, 이한샘, 김민아, 이기현

피인용수 : 0(자료제공 : 네이버학술정보)

초록

영어

Purpose: In this study, the neuroprotective effect of the methanol extract of Daphne genkwa Flos (DGF-ex) against glutamate-induced neurotoxicity was studied in mouse hippocampal HT22 cells. Materials and Methods: We conducted the cytoprotective effect of DGF-ex against a 24-hour-stress-induced condition by glutamate in HT22 cells. The inhibitory effect of DGF-ex on cellular necrosis and apoptosis and ROS (reactive oxygen species) was observed using fluorescence microscopy. To further clarify its antioxidant efficacy, assays on SOD (superoxide dismutase) regulation and DPPH/ABTS radical scavenging ability were also conducted. A chemical profile of DGF-ex was also obtained via UPLC analysis. Results: Pretreatment with DGF-ex significantly reduced glutamate-induced loss of cell viability and release of LDH (lactate dehydrogenase). DGF-ex showed similar effects to the antioxidant quercetin (15μM) in the evaluation of DPPH/ABST radical scavenging activity and SOD activity evaluation. In addition, DGF-ex remarkedly downregulated the glutamate-induced programmed HT22 cell death, which was shown in the normalization of the cellular necrosis and apoptosis ratio. Conclusion: These results exhibit DGF-ex has the ability to protect against glutamate-induced cytotoxicity in the HT22 cell line via the drug candidate’s antioxidant capacity. This research confirmed the DGF-ex as a valuable drug candidate for the prevention and treatment of oxidative stress-mediated neurodegenerative diseases.

목차

Abstract
서론
본론
1. 실험재료 및 방법
2. 실험결과 및 고찰
결론
감사의 글
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저자정보

  • 노경옥 Kyoung Ok No. 동신대학교 한의과대학 한의예과
  • 김경한 Kyeong-Han Kim. 우석대학교 한의과대학 예방의학교실
  • 이한샘 Han-Saem Lee. 한국한의약진흥원
  • 김민아 Min-A Kim. 한국한의약진흥원
  • 이기현 Gihyun Lee. 동신대학교 한의과대학 한의예과

참고문헌

자료제공 : 네이버학술정보

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