원문정보
초록
영어
The role of transient receptor potential vanilloid receptor-1 (TRPV1) has been primarily investigated in pain sensory neurons. Relatively, little research has been performed in testicular cells. TRPV1 is abundantly expressed in Leydig cells of young adult mice. This study was conducted to determine the role of the TRPV1 channel in Leydig cells. TRPV1 modulators and testosterone were treated to the mouse Leydig cell line TM3 cells for 24 h. Capsaicin, a TRPV1 activator, dose-dependently induced cell death, whereas capsazepine, a TRPV1 inhibitor, inhibited capsaicin-induced cell death. Testosterone treatment reduced capsaicin-induced cell death. High concentrations of testosterone decreased TRPV1 mRNA and protein expression levels. However, TRPV1 modulators did not affect testosterone production. These results showed that capsaicin induced cell death of Leydig cells and that testosterone reduced capsaicininduced cell death. Our findings suggest that testosterone may regulate the survival of Leydig cells in young adult mice by decreasing the expression level of TRPV1.
목차
INTRODUCTION
MATERIALS AND METHODS
Chemicals
Animals and testis isolation
Cell culture
Cell viability assay
Transfection
Annexin V/propidium iodide (PI) staining
Hematoxylin and eosin (H&E) staining
Immunohistochemistry (IHC)
TUNEL staining
Isolation of total RNA and reverse transcriptase-polymerase chain reaction (RT-PCR)
Western blot analysis
Measurement of testosterone concentration
Statistical analysis
RESULTS
Expression of TRPV1 in Leydig cells in testis of young mice
Capsaicin-induced cell death
Testosterone-induced decrease in TRPV1 expression
DISCUSSION
CONCLUSION
CONFLICTS OF INTEREST
ACKNOWLEDGEMENTS
AUTHOR CONTRIBUTIONS
AUTHOR’S POSITION AND ORCID NO.
REFERENCES