Research Article

자생 Tuber himalayense 자실체 추출물의 항염증 활성


Anti-inflammatory activity of indigenous Tuber himalayense in Korea

김민경, 홍혜현, 김정환, 김승영, 김창무

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In this study, the anti-inflammatory activity of an extract of the fruiting body of the Tuber himalayense (TH) truffle collected from oak growing areas in Korea was investigated. The extract was not cytotoxic at concentrations below 100 μg/mL in an experiment evaluating inflammation inhibitory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPSinduced nitric oxide (NO) and prostaglandin E2 (PGE2) production was inhibited by the extract in a concentration-dependent manner. Western blot assay results indicated that the anti-inflammatory activity of TH extract was likely caused by the reduced production of NO and PGE2 via suppression of induced NO synthase and cyclooxygenase-2 gene expression. In addition, TH extract effectively inhibited the production of interleukin (IL)-1β and IL-6 by macrophages. Thus, TH extract effectively inhibits the overexpression of various inflammatory mediators and could be valuable in formulating anti-inflammatory foods and medicines that target these components.


서 론
재료 및 방법
채집 표본의 분류 및 동정
자실체 열수 추출을 통한 분석용 시료 확보
분석용 대식세포의 준비
세포 생존율 측정
Nitric oxide(NO) 생성 억제 활성 측정
Prostaglandin E2(PGE2) 생성 억제 활성 측정
Pro-inflammatory cytokine(TNF-α, IL-6, IL-1β) 생성억제 활성 측정
결과 및 고찰
채집 표본의 분류 및 동정
세포 생존율 측정
NO 생성 억제 활성 측정
Prostaglandin E2 (PGE2) 생성 억제 활성 측정
iNOS 및 COX-2 발현 억제 활성 측정
Pro-inflammatory cytokine(TNF-α, IL-6, IL-1β) 생성억제 활성 측정
적 요


  • 김민경 Minkyeong Kim. 국립생물자원관 미생물자원과
  • 홍혜현 Hyehyun Hong. 선문대학교 제약생명공학과
  • 김정환 Jung-Hwan Kim. 선문대학교 제약생명공학과
  • 김승영 Seung-Young Kim. 선문대학교 제약생명공학과
  • 김창무 Changmu Kim. 국립생물자원관 유용자원분석과


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