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Purpose: In this study, an attempt was made to identify the extent of dental technicians’ perception of professionalism and examine to what extent the perception of professionalism impacts self-development and professionalism improvement activities. Methods: An online survey was conducted for 391 dental technicians for 20 days (i.e., from October 20 to November 10, 2020). Pearson’s correlation analysis was performed to examine the correlation between the independent variable and the dependent variable, and multiple regression analysis was performed to analyze the degree of influence of independent variables on self-development and professionalism improvement activities. Results: As a result of examining the impact of dental technicians’ perception of professionalism on self-development and professionalism improvement activities, it was revealed that all four subareas of the perception of professionalism, i.e., professional qualification, professional autonomy, social perception, and the sense of calling, were positively impacted. Conclusion: (1) It is necessary to compare and analyze the differences in the perception of professionalism among various dental technologist groups, prepare programs to improve them, and endeavor to ensure the growth of organizational operating system. (2) Self-development and professionalism improvement activities need to be supported so that dental technologists can immerse themselves in their duties with affections for the organization and contemplate and provide educational programs to workers.