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Objectives: This study explored the mediating effect of occupational stress between work-life balance and Professional Quality of Life (ProQOL) among nurses in local hospitals. Methods: A cross-sectional study was conducted through surveying hospital nurses (N=208). The collected data were analyzed using descriptive statistics, analysis of variance, Pearson correlation coefficient analysis, and a three step regression analysis using Baron and Kenny’s method for mediation. Results: Mean scores were 2.89±0.64, 2.96±0.49 and 2.58±0.59 for compassion satisfaction, burnout and secondary traumatic stress, respectively. Occupational stress had a partial mediating effect between work-life balance and compassion satisfaction as well as an explanatory power of 21.9%. Occupational stress had a partial mediating effect between work-life balance and burnout as well as an explanatory power of 52.8%. Conclusions: To improve nurses’ professional quality of life in local hospitals, intervention strategies for reducing occupational stress and promoting work-life balance should be considered.