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Regular Article

Effect of Plant Growth Regulators in In Vitro Culture of Hippophae rhamnoides

초록

영어

This study was carried out to establish in vitro propagation system influenced by plant growth regulators through organogenesis with three different seed sources (China, Mongolia and Russia) for conservation of genetic resources in Northeast Asia. The experiment compared two different carbon sources (commercial sugar, sucrose), which showed no significant differences in germination rate. Induced adventitious buds from leaf segments were found to be highly effective when supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin, and 5.0 mg/L IAA, in the case of Chinese origin 96.8%, Russian origin R-1: 95.6%, R-2: 85.6%, and Mongolian origin M-2: 77.8%. It was effective in BA and Kinetin with supplemented with IAA, respectively. Shooting development was also efficient in Woody Plant Media (WPM) supplemented with 1.0 mg/L BA, 1.0 mg/L Kinetin and 5.0 mg/L IAA.

목차

Abstract
Introduction
Materials and Methods
Experiment materials
Seed germination by the different carbon sources
Adventitious bud induction by the treatment of plant growth regulators
Root induction
Results and Discussion
Morphology of seabuckthorn seeds
Seed germination by the different carbon sources
Adventitious bud induction by the treatment plant growth regulators
Root induction
Acknowledgements
References

저자정보

  • Songhee Lee Department of Biological and Environmental Science, Dongguk University, Goyang 10326, Republic of Korea
  • Wonwoo Cho Department of Forest Bioresources, National Institute of Forest Science, Suwon 16631, Republic of Korea
  • Hyeonsoo Jang Crop Production & Physiology Div., National Institute of Crop Science, Wanju 55365, Republic of Korea
  • Romika Chandra Department of Biological and Environmental Science, Dongguk University, Goyang 10326, Republic of Korea
  • Sora Lee Forest Biomaterials Research Center, National Institute of Forest Science, Jinju 52817, Republic of Korea
  • Hoduck Kang Department of Biological and Environmental Science, Dongguk University, Goyang 10326, Republic of Korea

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