원문정보
초록
영어
Purpose: It has been reported that the diversity and abundance of microbes in the vagina decrease due to the use of antimicrobial agents, and the high recurrence rate of female vaginitis due to this suggests that a new treatment is needed. Methods: In the experiment, we detected that 10% potassium sorbate solution, 1% eucalyptus oil solution, 1% tea tree oil solution, 400 μL/10 mL grapefruit seed extract solution, 100% lactic acid, 10% acetic acid solution, and 10% lactic acid solution were prepared and used. After adjusting the pH to 4, 5, and 6 with lactic acid and acetic acid in the mixed culture medium, each bacterium was inoculated into the medium and incubated for 72 h at 35°C. Incubate and 0 h each. 24 h. 48 h. The number of bacteria was measured after 72 h. Results: In the mixed culture test between lactic acid bacteria and pathogenic microorganisms, lactic acid bacteria showed good results at pH 5–5.5. Potassium sorbate, which has varying antibacterial activity based on the pH, killed pathogenic bacteria and allowed lactic acid bacteria to survive at pH 5.5. Conclusion: The formulation ratio obtained through this study could be used for the development of a feminine cleanser that can be used as a substitute for antibacterial agents. Further, the findings of this study may be able to solve the problem of antimicrobial resistance in the future.
목차
1. Introduction
2. Experiments
2.1 Materials
2.2 Methods
3. Results
3.1 Comparison of Bacterial Growth in Mixed Culture with Appropriate pH in the Presence of Lactic Acid and Acetic Acid
3.2 Comparison of the Growth of L. plantarum, C. albicans, and E. coli in the Presence of Potassium Sorbate
3.3 Comparison of the Growth of L. plantarum, C. albicans, and E. coli in a Mixed Solution of Grapefruit Seed Extract, Eucalyptus Oil, and Tea Tree Oil
3.4 Comparison of the Growth of L. plantarum, C. albicans, and E. coli in a Mixed Solution of Potassium Sorbate, Grapefruit Seed Extract, Eucalyptus Oil, and Tea Tree Oil
3.5 Comparison of Str. thermophilus, L. plantarum, C. albicans, and E. coli Growth with a Potassium Sorbate, Grapefruit Seed Extract, Eucalyptus Oil, and Tea Tree Oil Mixture at pH 5.0, 5.5, and 6.0
4. Discussion
5. Conclusion
Acknowledgement
References