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Background/Objectives: The fire related injuries such as burns are one of the main problems of the community. It is because of the insufficient ideas in wound healing and lack of financial capabilities resulting into self-medication. Methods/Statistical analysis: In this study, the researchers will prepare an ointment from milkfish skin and scale added to soft paraffin in 70 C until it congeals. The milkfish is known having high content of riboflavin and vitamin B complex that is a big impact to fasten the first-degree burn healing process. The milkfish skin and scales are also to be extracted using Gamma Irradiation as a manner of sterilization and for preservation of collagen and vitamin B content. The experimental rats will be induced of burn wounds by 5 seconds in the back part using cylindrical heat steel to be associated in 100C boiling water which will be treated with the prepared ointment. The epithelialization times, wound contraction and histological examination will be observed and the data recorded is to be analyzed using one-way ANOVA and repeated measures. Findings: This study deals with scientific experimentation to identify the potential of Milkfish skin and scale in wound healing. Improvements/Applications: If proven effective, it may be utilized as an alternative wound healing agent.