earticle

영어

A goat that had been grazing was found dead. Autopsy found needle-like substances presumed to be yew leaves in the stomach, and yew ingestion was suspected. In the screening using gas chromatography-mass spectrometry(GC–MS), 3,5-dimethoxyphenol, the aglycone of taxicatine which is a typical ingredient of yew leaves, was identified as indirect evidence of yew ingestion, and toxicological analysis for compounds of yew, taxoids, was performed based on liquid–liquid extraction, followed by liquid chromatography–quadrupole time-of-flight mass spectrometry(LC–QTOF–MS) without reference standards. Using the analytical method, we determined six taxoids, namely taxine B, cephalomannine, paclitaxel, 10-deacetyltaxol (10-DAT), baccatin III, and 10-deacetylbaccatin III(10-DAB III), in the stomach contents. Based on these results of toxicological analysis, especially the determination of taxine B, and morphological finding, yew poisoning was proven as the cause of death.